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cell staining reagents  (Beyotime)


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    Structured Review

    Beyotime cell staining reagents
    Cell Staining Reagents, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining+reagent/DAPI/pmc13098730-37-0-14
    Average 99 stars, based on 32249 article reviews
    cell staining reagents - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: A novel 68 Ga-labeled cyclic peptide molecular probe based on the computer-aided design for noninvasive imaging of PD-L1 expression in tumors.
    Article Snippet: Programmed death-ligand 1 (PD-L1) serves as a crucial biomarker for guiding the screening of cancer patients and the stratification of immunotherapy.. However, due to the high heterogeneity of tumors, the current gold standard for detecting PD-L1 expression (immunohistochemistry) fails to comprehensively evaluate the overall PD-L1 expression levels in the body.. Fortunately, the use of PD-L1 targeted radiotracers enables quantitative, real-time, and noninvasive assessment of PD-L1 expression levels and dynamics in tumors.

    Article Title: Imaging and Monitoring HER2 Expression in Tumors during HER2 Antibody-Drug Conjugate Therapy Utilizing a Radiolabeled Site-Specific Single-Domain Antibody Probe: 68 Ga-NODAGA-SNA004-GSC.
    Article Snippet: The overexpression of HER2 is pivotal in the initiation and progression of breast cancer.. Developing HER2-targeted radiotracers is crucial for noninvasive assessment of HER2 expression, patient selection for HER2-targeted therapy, monitoring treatment response, and identifying resistance.. Here, we reported a nonsite-specific coupled radiotracer, Ga-NOTA-SNA004-His6, and a site-specific coupled radiotracer, 68Ga-NODAGA-SNA004-GSC, based on a novel HER2 nanobody, SNA004.

    Article Title: LncRNA FGD5-AS1 Promotes Abdominal Aortic Aneurysm Growth Through the Activation of MMP3 in Vascular Smooth Muscle Cells.
    Article Snippet: TUNEL staining: After appropriate treatment, VSMCs in each experimental group were immobilized with paraformaldehyde for 1 hour, and apoptotic cells were labeled with In Situ Cell Death Detection Kit (TMR-red) (Roche, Switzerland). .. DAPI staining reagent (Beyotime, China) was used for nuclear staining. ..

    Article Title: Fe-flavonoid nanozyme as dual modulator of oxidative stress and autophagy for acute kidney injury repair
    Article Snippet: Minimum essential medium (MEM; PM150410) and Dulbecco's modified Eagle's medium (DMEM; PM150210) were purchased from Pricella (Wuhan, China). .. Paraformaldehyde (P0099), penicillin-streptomycin (C0222), trypsin (C0201), DAPI staining reagent (C1002), Cell Counting Kit-8 (CCK-8) (C0037), JC-1 dye (C2006), calcein/propidium iodide staining and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) kit (P0012A) were purchased from Beyotime (Shanghai, China). .. N-acetylcysteine (NAC) was obtained from Solarbio (Beijing, China).

    Article Title: MSCs-derived ECM functionalized hydrogel regulates macrophage reprogramming for osteoarthritis treatment by improving mitochondrial function and energy metabolism
    Article Snippet: .. Afterward, the secondary antibodies, CoraLite®594 (1:500) (Proteintech, China) and CoraLite®488 (1:500) (Proteintech, China), were incubated at 37 °C for 1 h. The cell nuclei were stained with DAPI staining reagent (Beyotime, China). .. The samples were observed and imaged using a confocal laser scanning microscope (Nikon, Japan).

    Article Title: Kaempferol alleviates high-fat-induced hepatocyte injury by inhibiting ferroptosis.
    Article Snippet: Background: Metabolic dysfunction-associated steatotic liver disease (MASLD) is a key manifestation of metabolic syndrome in the liver.. Its core pathological features are excessive lipid accumulation in hepatocytes and the subsequent lipotoxic damage.. This not only drives the progression of the disease to hepatitis, liver fibrosis, and cirrhosis but also is significantly associated with an increased risk of hepatocellular carcinoma.

    Article Title: HOXC8 alleviates high glucose-triggered damage of trophoblast cells during gestational diabetes mellitus via activating TGFβ1-mediated Notch1 pathway.
    Article Snippet: Gestational diabetes mellitus (GDM) is an increasingly frequent disease occurred during pregnancy.. HOXC8 has been disclosed to take part in the regulation of cancers.. Additionally, the HOXC8 expression was dramatically decreased in the placenta of pre-eclampsia patients, but its expression and function have not been investigated in GDM.

    Labeling:

    Article Title: Imaging and Monitoring HER2 Expression in Tumors during HER2 Antibody-Drug Conjugate Therapy Utilizing a Radiolabeled Site-Specific Single-Domain Antibody Probe: 68 Ga-NODAGA-SNA004-GSC.
    Article Snippet: The overexpression of HER2 is pivotal in the initiation and progression of breast cancer.. Developing HER2-targeted radiotracers is crucial for noninvasive assessment of HER2 expression, patient selection for HER2-targeted therapy, monitoring treatment response, and identifying resistance.. Here, we reported a nonsite-specific coupled radiotracer, Ga-NOTA-SNA004-His6, and a site-specific coupled radiotracer, 68Ga-NODAGA-SNA004-GSC, based on a novel HER2 nanobody, SNA004.

    CCK-8 Assay:

    Article Title: Fe-flavonoid nanozyme as dual modulator of oxidative stress and autophagy for acute kidney injury repair
    Article Snippet: Minimum essential medium (MEM; PM150410) and Dulbecco's modified Eagle's medium (DMEM; PM150210) were purchased from Pricella (Wuhan, China). .. Paraformaldehyde (P0099), penicillin-streptomycin (C0222), trypsin (C0201), DAPI staining reagent (C1002), Cell Counting Kit-8 (CCK-8) (C0037), JC-1 dye (C2006), calcein/propidium iodide staining and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) kit (P0012A) were purchased from Beyotime (Shanghai, China). .. N-acetylcysteine (NAC) was obtained from Solarbio (Beijing, China).

    Article Title: Kaempferol alleviates high-fat-induced hepatocyte injury by inhibiting ferroptosis.
    Article Snippet: Background: Metabolic dysfunction-associated steatotic liver disease (MASLD) is a key manifestation of metabolic syndrome in the liver.. Its core pathological features are excessive lipid accumulation in hepatocytes and the subsequent lipotoxic damage.. This not only drives the progression of the disease to hepatitis, liver fibrosis, and cirrhosis but also is significantly associated with an increased risk of hepatocellular carcinoma.

    Nucleic Acid Electrophoresis:

    Article Title: Fe-flavonoid nanozyme as dual modulator of oxidative stress and autophagy for acute kidney injury repair
    Article Snippet: Minimum essential medium (MEM; PM150410) and Dulbecco's modified Eagle's medium (DMEM; PM150210) were purchased from Pricella (Wuhan, China). .. Paraformaldehyde (P0099), penicillin-streptomycin (C0222), trypsin (C0201), DAPI staining reagent (C1002), Cell Counting Kit-8 (CCK-8) (C0037), JC-1 dye (C2006), calcein/propidium iodide staining and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) kit (P0012A) were purchased from Beyotime (Shanghai, China). .. N-acetylcysteine (NAC) was obtained from Solarbio (Beijing, China).

    Incubation:

    Article Title: MSCs-derived ECM functionalized hydrogel regulates macrophage reprogramming for osteoarthritis treatment by improving mitochondrial function and energy metabolism
    Article Snippet: .. Afterward, the secondary antibodies, CoraLite®594 (1:500) (Proteintech, China) and CoraLite®488 (1:500) (Proteintech, China), were incubated at 37 °C for 1 h. The cell nuclei were stained with DAPI staining reagent (Beyotime, China). .. The samples were observed and imaged using a confocal laser scanning microscope (Nikon, Japan).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Kaempferol alleviates high-fat-induced hepatocyte injury by inhibiting ferroptosis.
    Article Snippet: Background: Metabolic dysfunction-associated steatotic liver disease (MASLD) is a key manifestation of metabolic syndrome in the liver.. Its core pathological features are excessive lipid accumulation in hepatocytes and the subsequent lipotoxic damage.. This not only drives the progression of the disease to hepatitis, liver fibrosis, and cirrhosis but also is significantly associated with an increased risk of hepatocellular carcinoma.

    TUNEL Assay:

    Article Title: HOXC8 alleviates high glucose-triggered damage of trophoblast cells during gestational diabetes mellitus via activating TGFβ1-mediated Notch1 pathway.
    Article Snippet: Gestational diabetes mellitus (GDM) is an increasingly frequent disease occurred during pregnancy.. HOXC8 has been disclosed to take part in the regulation of cancers.. Additionally, the HOXC8 expression was dramatically decreased in the placenta of pre-eclampsia patients, but its expression and function have not been investigated in GDM.

    Double Staining:

    Article Title: HOXC8 alleviates high glucose-triggered damage of trophoblast cells during gestational diabetes mellitus via activating TGFβ1-mediated Notch1 pathway.
    Article Snippet: Gestational diabetes mellitus (GDM) is an increasingly frequent disease occurred during pregnancy.. HOXC8 has been disclosed to take part in the regulation of cancers.. Additionally, the HOXC8 expression was dramatically decreased in the placenta of pre-eclampsia patients, but its expression and function have not been investigated in GDM.

    other:

    Article Title: A cutting-edge 68 Ga-labeled bicyclic peptide PET molecular probe for noninvasive assessment of Nectin4 expression.
    Article Snippet: The diagnosis and treatment of triple negative breast cancer (TNBC) are huge challenges due to the lack of identifiable molecular targets.. The high expression of Nectin4 in a variety of tumors, including TNBC, is associated with the occurrence, invasion, progression and poor prognosis of tumors.. Therefore, Nectin4 is an emerging biomarker for the diagnosis and treatment of TNBC.



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